Measurements should be taken with the inner border of the horizontal line of the callipers placed ON the line that defines the nuchal translucency thickness - the crossbar of the calliper should be such that it is hardly visible as it merges with the white line of the border, not in the nuchal fluid.The widest part of translucency must always be measured.
Care must be taken to distinguish between fetal skin and amnion. When the fetal neck is hyperextended the measurement can be falsely increased and when the neck is flexed, the measurement can be falsely decreased. The fetus should be in a neutral position, with the head in line with the spine. Minor deviations from the exact midline plane would cause non-visualization of the tip of the nose and visibility of the maxilla. 
This is defined by the presence of the echogenic tip of the nose and rectangular shape of the palate anteriorly, the translucent diencephalon in the centre and the nuchal membrane posteriorly.
A mid-sagittal view of the face should be obtained. The magnification of the image should be such that the fetal head and thorax occupy the whole screen. The fetal crown-rump length should be between 45 and 84mm. The gestational period must be 11 to 13 weeks and six days. Submission of a logbook of 3 images demonstrating the measurement of NT. Attendance of the internet based course on the 11-13 weeks scan. The requirements for obtaining the FMF certificate of competence in the nuchal translucency (NT) scan are: High nuchal translucency Requirements for certification Should they wish to use the additional markers for chromosomal abnormalities (nasal bone, tricuspid flow and ductus venosus flow) they would need to obtain the appropriate FMF Certificate for each of these markers. Sonographers who have obtained the FMF Certificate in the nuchal translucency (NT) scan can obtain free of charge the FMF software for first-trimester screening for chromosomal abnormalities by maternal age, fetal NT thickness and maternal serum free β-hCG and PAPP-A. Appropriate counselling of the parents. The software companies that use this algorithm are Astraia Software GmbH, ViewPoint GE Healthcare and Zeitgeist Health SE. 
A risk calculation program that uses an algorithm based on scientific evidence. Measurement of maternal serum free β-hCG and PAPP-A by laboratories that can demonstrate good quality assurance performance. Carrying out the ultrasound examination by appropriately trained sonographers. The FMF advocates that effective screening requires: The Fetal Medicine Foundation (FMF) has introduced a process of comprehensive training, support and audit for the proper implementation of early screening for chromosomal abnormalities. Early screening for chromosomal abnormalities
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